Gram-Positive and Acid-Fast Bacteria Coursework

Gram-Positive and Acid-Fast Bacteria - Coursework Example Name and describe the stages of bacterial growth. How would you keep a culture in the stationary phase?Lag phase- at this stage the bacteria gets to adopt them to the growth condition. They undergo maturation, but they are not capable of dividing.Log phase- the phase if characterized by cell multiplication. Normally, the number of new bacteria per unit time is proportional to the current population. The stationary phase is premised on growth inhibiting factors.Stationary phase- It is the stage where growth rate is proportional to the death rate.Death phase- It is the stage where the bacteria finally die due to adverse living conditions that are brought about by the limiting factors.A culture can be kept in the stationary phase by invoking growth inhibiting factors such as high/low temperatures as well as limiting the amount of nutrients available for the bacteria.4. Describe the different sterilization techniques and know when to use each type. (For example, when would you use an aut oclave, gas, etc.?)Steam autoclave- it combines moisture and high temperatures to kill microorganisms. The technique utilizes temperature ranging from 121-148 degrees Celsius at a pressure of 15 P.S.I. Typically, the sterilization period is dependent on the load and temperature but can vary from ten minutes to one hour. It is used for surgical equipment.Chemical sterilization- The method utilized chemical agents that have the ability to kill microorganism for instance ethylene oxide gas. It is a low-temperature sterilization process.